教員情報詳細

写真：原清敬

氏名: 原清敬（HARA Kiyotaka）

出生年月: 1974年8月

所属・職名: 食品栄養科学部環境生命科学科（環境工学研究室）准教授
食品栄養環境科学研究院　准教授（兼務）

電話番号: 054-264-5659

部屋番号: 食品栄養科学部2号棟203号室

Eメールアドレス: k-hara@u-shizuoka-ken.ac.jp

ホームページアドレス（URL）: http://sweb.u-shizuoka-ken.ac.jp/~env-bioeng/

researchmap（URL）: https://researchmap.jp/read0210028

研究シーズ集: https://www.u-shizuoka-ken.ac.jp/media/seeds2022shoku-028.pdf

学歴

1997年3月　東京工業大学生命理工学部生体機構学科卒業
2001年9月　東京工業大学院総合理工学研究科博士課程修了

学位

博士（理学）（東京工業大学・2001年）

専門分野

生化学、応用微生物学、生物工学、合成生物学

担当科目

環境科学入門、環境工学、基礎環境生命科学実験、環境生命科学実験I、環境生命科学特論、環境工学特論、基礎生化学、合成生物学、フィールドワーク演習

主要研究テーマ

微生物を用いた再生可能資源(バイオマス)からの有用物質生産
微生物の細胞内エネルギー向上による有用物質生産性向上
微生物を用いた機能性化合物（食品・飼料・肥料など）の発酵生産

所属学会

日本生化学会
日本ゲノム微生物学会
日本生物工学会
日本農芸化学会
日本化学工学会
日本環境化学会

主な経歴

2002年4月　日本科学技術振興事業団・博士研究員
2003年5月　協和発酵工業株式会社・研究員
2006年3月　早稲田大学・特任助手
2009年3月　神戸大学・特命助教
2012年4月　神戸大学・特命准教授
2015年6月　静岡県立大学・准教授

主な社会活動

科学技術予測センター専門調査員
日本生物工学会　英文誌編集委員

主要研究業績

○原著論文

Hirono-Hara Y, Yui M, Hara KY (2021) Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae. AMB Express. 11:13.
Hara KY, Kageyama Y, Tanzawa N, Hirono-Hara Y, Kikukawa H, Wakabayashi K (2020) Development of astaxanthin production from citrus peel extract using Xanthophyllomyces dendrorhous, Environ. Sci. Pollut. Res. doi: 10.1007/s11356-020-11163-7.
Imai Y, Inoshita T, Meng H, Shiba-Fukushima K, Hara KY, Sawamura N, Hattori N. (2019) Light-driven activation of mitochondrial proton-motive force improves motor behaviors in a Drosophila model of Parkinson's disease. Commun Biol. 2:424.
Hara KY, Saito M, Kato H, Morikawa K, Kikukawa H, Nomura H, Fujimoto T, Hirono-Hara Y, Watanabe S, Kanamaru K, Kondo A. (2019) 5-Aminolevulinic acid fermentation using engineered Saccharomyces cerevisiae. Microb Cell Fact. 18 (1):194.
Kawaguchi H, Yoshihara K, Hara KY, Hasunuma T, Ogino C, Kondo A. (2018) Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose. Microb Cell Fact. 17(1):76.
Prima A, Hara KY, Djohan AC, Kashiwagi N, Kahar P, Ishii J, Nakayama H, Okazaki F, Prasetya B, Kondo A, Yopi, Ogino C. (2017) Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae. Bioresour Technol. 245(Pt B):1400-1406.
Kobayashi J, Sasaki D, Hara KY, Hasunuma T, Kondo A. (2017) Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae. Microb Cell Fact, 16(1):44.
Yamamoto K, Hara KY, Morita T, Nishimura A, Sasaki D, Ishii J, Ogino C, Kizaki N, Kondo A. (2016) Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes. Microb Cell Fact. 15(1):155.
Ishii J, Okazaki F, Djohan AC, Hara KY, Asai-Nakashima N, Teramura H, Andriani A, Tominaga M, Wakai S, Kahar P, Yopi, Prasetya B, Ogino C, Kondo A. (2016) From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae. Biotechnol Biofuels. 9(1):188.
Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. (2016)Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 353 (6305).
Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A. (2016) Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae. J Biosci Bioeng., 121, 96-100.
Hara KY, Aoki N, Kobayashi J, Kiriyama K, Nishida K, Araki M, Kondo A. (2015) Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae. Appl Microbiol Biotechnol., 99, 9771-9778.
Inokuma K, Ishii J, Hara KY, Mochizuki M, Hasunuma T, Kondo A. (2015) Complete Genome Sequence of Kluyveromyces marxianus NBRC1777, a Nonconventional Thermotolerant Yeast. Genome Announc. 3(2).
Ye X, Morikawa K, Ho SH, Araki M, Nishida K, Hasunuma T, Hara KY (Corresponding author), Kondo A. (2015) Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity. Microb Cell Fact 14, 56.
Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, and Akihiko Kondo (2015) Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse. Bioresource Technology, 182, 169-178.
Kiyotaka Y. Hara, Toshihiko Morita, Masao Mochizuki, Keisuke Yamamoto, Chiaki Ogino, Michihiro Araki, Akihiko Kondo (2014) Development of a multi-gene expression system in Xanthophyllomyces dendrorhous. Microbial Cell Factories 13(1), 175.
Michihiro Araki, Robert Sidney Cox III, Hiroki Makiguchi, Teppei Ogawa, Takeshi Taniguchi, Kohei Miyaoku, Masahiko Nakatsui, Kiyotaka Y. Hara, Akihiko Kondo (2014) M-path: a compass for navigating potential metabolic pathways. Bioinformatics. 31(6), 905-911.
Kiyotaka Y. Hara, Katsuhiko Morita, Yusuke Endo, Masao Mochizuki, Michihiro Araki, Akihiko Kondo (2014) Evaluation and screening of efficient promoters to improve astaxanthin production in Xanthophyllomyces dendrorhous. Applied Microbiology and Biotechnology 98(15), 6787-6793.
Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Kiyotaka Hara, Tomohisa Hasunuma, Akihiko Kondo (2014) Optimizing biodiesel production in marine Chlamydomonas sp.JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Biotechnology for Biofuel 7, 97.
Kentaro Kiriyama, Kiyotaka Y. Hara (equal contribution), Akihiko Kondo (2013) Oxidized glutathione fermentation using Saccharomyces cerevisiae engineered for glutathione metabolism. Applied Microbiology and Biotechnology 97(16), 7399-7404.
Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura (2013) Construction of photoenergetic mitochondria in cultured mammalian cells. Scientific Reports 3, 1635.
Kentaro Kiriyama, Kiyotaka Y. Hara (equal contributin), Akihiko Kondo (2012) Extracellular glutathione fermentation using engineered Saccharomyces cerevisiae expressing novel glutathione exporter. Applied Microbiology and Biotechnology 96(4), 1021-1027.
Jun Ishii, Miyuki Moriguchi, Kiyotaka Y. Hara, Seiji Shibasaki, Hideki Fukuda, Akihiko Kondo (2012) Improved identification of agonist-mediated Gαi-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry. Analytical Biochemistry 426(2), 129-133.
Kiyotaka Y. Hara, Songhee Kim, Kentaro Kiriyama, Hideyo Yoshida, Syogo Arai, Jun Ishii, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2012) An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae. Biotechnology Journal 7(5), 686-689.
Kiyotaka Y. Hara, Kentaro Kiriyama, Hideo Nakayama, Akihiko Kondo (2012) Improvement　of glutathione production by metabolic engineering the sulfate assimilation pathway of Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 94(5), 1313-1319.
Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai, N, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2012) Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 93(4), 1495-1502.
Hideyo Yoshida, Kiyotaka Y. Hara (equal contribution), Kentro Kiriyama, Hideo Nakayama, Fumio Matsuda,Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2011) Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst., Applied Microbiology and Biotechnology 91(4), 1001-1006.
Kiyotaka Y. Hara, Rie Suzuki, Toshiharu Suzuki, Masasuke Yoshida, Kuniki Kino (2011) ATP photosynthetic vesicles for light-driven bioprocesses. Biotechnology Letters 33(6), 1133-1138.
Hideyo Yoshida, Shogo Arai, Kiyotaka Y. Hara, Ryosuke Yamada, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2011) Efficient and direct glutathione production from raw starch using Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 89(5), 1417-1422.
Kiyotaka Y. Hara, Natsuka Shimodate, Yasutaka Hirokawa, Mikito Ito, Tomoya Baba, Hirotada Mori, Hideo Mori (2009) Glutathione production by efficient ATP-regenerating Escherichia coli mutants. FEMS Microbiology Letters 297(2), 217-214.
Kiyotaka Y. Hara, Natsuka Shimodate, Mikito Ito, Tomoya Baba, Hirotada Mori, Hideo Mori (2009) Systematic genome-wide scanning for genes involved in ATP generation in Escherichia coli. Metabolic Engineering 11(1), 1-7.
Kiyotaka Y. Hara, Hideo Mori (2006) An efficient method for quantitative determination of cellular ATP synthetic activity. Journal of Biomolecular Screening 11(3), 310-317.
Katsuya Shimabukuro, Ryohei Yasuda, Eiro Muneyuki, Kiyotaka Y. Hara, Kazuhiko Kinosita, Jr, Masasuke Yoshida (2003) Catalysis and rotation of F1 motor: cleavage of ATP at the catalytic site occurs in 1 ms before 40 degree substep rotation. Proceedings of National Academy of Sciences of the United States of America 100(25),14731-14736.
Sakurako Ono, Kiyotaka Y. Hara, Jun Hirao, Tadashi Matsui, Hiroyuki Noji, Masasuke Yoshida, Eiro Muneyuki (2003) Origin of apparent negative cooperativity of F(1)-ATPase., Biochimica et Biophysica Acta -Bioenergetics 1607(1), 35-44.
Yoko Hirono-H, Hiroyuki Noji, Masaya Nishiura, Eiro Muneyuki, Kiyotaka Y. Hara, Ryohei Yasuda, Kazuhiko Kinosita, Jr, Masasuke Yoshida (2001) Pause and rotation of F(1)-ATPase during catalysis. Proceedings of National Academy of Sciences of the United States of America 98(24),13649-13654.
Kiyotaka Y. Hara, Yasuyuki Kato-Yamada, Yuji Kikuchi, Toru Hisabori, Masasuke Yoshida (2001) The role of the betaDELSEED motif of F1-ATPase: propagation of the inhibitory effect of the epsilon subunit. Journal of Biological Chemistry 276(26), 23969-23973.
Kiyotaka Y. Hara, Hiroyuki Noji, Dirk Bald, Ryohei Yasuda, Kazuhiko Kinosita, Jr., Masasuke Yoshida (2000) The role of the DELSEED motif of the beta subunit in rotation of F1-ATPase. Journal of Biological Chemistry 275(19), 14260-14263.
Toyoki Amano, Tadashi Matsui, Eiro Muneyuki, Hiroyuki Noji, Kiyotaka Hara, Masasuke Yoshida, Toru Hisabori (1999) alpha3beta3gamma complex of F1-ATPase from thermophilic Bacillus PS3 can maintain steady-state ATP hydrolysis activity depending on the number of non-catalytic sites. Biochemical Journal 343(1), 135-138.

○総説

Hara KY, Kobayashi J, Yamada R, Sasaki D, Kuriya Y, Hirono-Hara Y, Ishii J, Araki M, Kondo A. (2017) Transporter engineering in biomass utilization by yeast. FEMS Yeast Res. 17(7).
Kiyotaka Y. Hara and Akihiko Kondo (2015) ATP regulation in bioproduction. Microb Cell Fact. 14(1), 198.
Kiyotaka Y. Hara, Michihiro Araki, Naoko Okai, Satoshi Wakai, Tomohisa Hasunuma, Akihiko Kondo (2014) Development of bio-based fine chemical production through synthetic bioengineering. Microbial Cell Factries 13(1), 173.
Tomohisa Hasunuma, Fumio Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo (2013) A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology. Bioresourse Technology 135, 513-522.
Akihiko Kondo, Jun Ishii, Kiyotaka Y. Hara, Tomohisa Hasunuma, Fumio Matsuda (2013) Development of microbial cell factories for bio-refinery through synthetic bioengineering. Journal of Biotechnololgy 163(2), 204-216.

○著書

原清敬　(2023) 『食品ロス削減と食品廃棄物資源化の技術』 13章微生物を用いた飲料抽出残渣からのファインケミカルの発酵生産技術の開発（CMC出版）
原清敬　(2016)「共生由来オルガネラの改変―共生が駆動する微生物発酵生産」『生物の科学　遺伝』（2016年3月号）特集　真核細胞の共生由来オルガネラ研究最前線―広がり続ける多様性と機能（NTS出版）70, 110-114
原清敬、近藤昭彦　(2014) 28章バイオマス利用技術化学便覧、日本化学会編、　応用化学編第7版（丸善出版）1615-1651（分担執筆）28.5.3節担当
Ye Xiaoting、原清敬　(2014)「もっと光を！人工光合成細菌の魅力」生物工学会誌　バイオミディア（日本生物工学会）92, 444（共著）
原清敬、下立夏香、森英郎 (2010) 「大腸菌ATP供給活性向上変異体の効率的探索と物質生産への応用」、バイオサイエンスとインダストリー（バイオインダストリー協会）68,190-193（共著）
Kiyotaka Y. Hara (2009) Methods in Molecular Biology: Methods and Protocols, Reverse Chemical Genetics (Section 2.7. Permeable Cell Assay: a method for high-throughput measurement of cellular ATP synthetic activity), Methods in Molecular Biology (Humana Press) 577, 251-257（分担執筆）
原清敬、吉田賢右 (2001)「モチーフドメインリスト」生体の科学（医学書院）52, 502-503（分担執筆）

教育・研究に対する考え方

教育・研究で養うべき７つの力として、①楽しむ力、②発想力、③思考力、④対話力、⑤応用力、⑥協力、⑦挑戦力が重要と思います。

研究シーズ集に関するキーワード

再生可能資源, バイオマス, バイオマスリファイナリー, バイオエコノミー, 光エネルギー利用, 発酵, スクリーニング, 合成生物（工）学, ファインケミカル

静岡県立大学教員データベース