グローバルナビゲーションへ

本文へ

ローカルナビゲーションへ

フッターへ



静岡県立大学教員データベース


教員情報詳細


氏名
轟木 堅一郎(TODOROKI Kenichiro)
出生年月
1973年9月
所属・職名
薬学部薬学科(生体機能分子分析学分野) 教授
薬学研究院(生体機能分子分析学教室) 教授(兼務)
電話番号
054-264-5656
部屋番号
薬学部棟6223号室
Eメールアドレス
todoroki@u-shizuoka-ken.ac.jp
ホームページアドレス(URL)
https://w3pharm.u-shizuoka-ken.ac.jp/analchem/
研究シーズ集
https://www.u-shizuoka-ken.ac.jp/media/seeds2019-2020_017.pdf

学歴

1996年3月 九州大学薬学部製薬化学科卒業
1998年3月 九州大学大学院薬学研究科薬学専攻博士課程(前期)修了
2001年3月 九州大学大学院薬学研究院薬学専攻博士課程(後期)満期退学

学位

博士(薬学)(福岡大学・2005年)

専門分野

分析化学

担当科目

分析化学Ⅰ、分析化学Ⅱ、薬品分析化学、自然科学概論、科学演習、物理系薬学実習、薬品分析学特論、病態分子分析学特論

主要研究テーマ

  • DNAアプタマーを利用するバイオアナリシス法の研究
  • オミクス解析のための新規分析ツールの開発
  • 抗体医薬品の新規体内動態解析法の開発
  • ポイントオブ検査を指向した薬物、バイオマーカーの新規分析法開発

所属学会

日本薬学会
日本分析化学会
日本化学会
クロマトグラフィー科学会(理事)
フルオラス科学研究会(評議員)
新アミノ酸分析研究会(副会長)
高分子学会

主な経歴

2001年4月 福岡大学薬学部助手
2007年4月 福岡大学薬学部助教
2010年4月 久留米バイオカレッジ非常勤講師(兼務)
2011年4月 静岡県立大学薬学部准教授
2017年4月 静岡県立大学薬学部教授

受賞歴

2008年 フィジカルファーマフォーラム奨励賞
2009年 クロマトグラフィー科学会奨励賞
2010年 日本薬学会九州支部奨励賞
2013年 Agilent Technologies Best Poster Award, 39th International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques (Amsterdam,全945演題中のTop10)
2016年 平成27年度静岡県立大学教員活動評価学長表彰
2019年 轟木堅一郎:静岡テックプラングランプリリアルテックファンド賞

主な社会活動

Chromatography誌Editor
クロマトグラフィー科学会理事
日本薬学会物理系薬学部会若手世話人
新アミノ酸分析研究会幹事
フルオラス科学研究会評議員

主要研究業績

○学術論文
  • "Bioanalytical methods for therapeutic monoclonal antibodies and antibody–drug conjugates: A review of recent advances and future perspectives." J. Pharm. Biomed. Anal. 112991 (2019)
  • "High-throughput bioanalysis of bevacizumab in human plasma based on enzyme-linked aptamer assay using anti-idiotype DNA aptamer" Anal. Chem. 91, 3125-3130 (2019)
  • "Isotope Corrected Chiral and Achiral Nontargeted Metabolomics: An Approach for High Accuracy and Precision Metabolomics Based on Derivatization and Its Application to Cerebrospinal Fluid of Patients with Alzheimer’s Disease." Anal. Chem. 91, 4396-4404 (2019)
  • "Anti-Idiotype DNA Aptamer Affinity Purification–High-Temperature Reversed-Phase Liquid Chromatography: A Simple, Accurate, and Selective Bioanalysis of Bevacizumab" Molecules, 24, 857 (2019)
  • "High-Temperature Reversed-Phase LC Separation of Heavy and Light Chain Fragments of Therapeutic Monoclonal Antibodies and Antibody-Drug Conjugate Produced by Chemical Reduction." Chromatography 40, 99-104 (2019)
  • "An accurate differential analysis of carboxylic acids in beer using ultra high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry based on chiral derivatization combining three isotopic reagents." Talanta, 205 120146 (2019)
  • "A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney." Mol. Imaging 18, 1536012118820421 (2019)
  • "Current mass spectrometric tools for the bioanalyses of therapeutic monoclonal antibodies and antibody-drug conjugates" Anal. Sci., 34 397-406 (2018)
  • “High Sensitivity and Precision High-Temperature Reversed-Phase LC Analysis of Bevacizumab for Intact Bioanalysis of Therapeutic Monoclonal Antibodies” Chromatography, 39, 21-26 (2018)
  • “Bioanalysis of bevacizumab and infliximab by high-temperature reversed-phase liquid chromatography with fluorescence detection after immunoaffinity magnetic purification” Anal. Chim. Acta, 916, 112-119 (2016).
  • “Advanced dress-up chiral columns: New removable chiral stationary phases for enantioseparation of chiral carboxylic acids” Anal. Chim. Acta, 882, 101-111 (2015).
  • “4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride as an enantioseparation enhancer for chiral derivatization-LC analysis of DL-amino acids” Chromatography, 37, 23-28 (2016).
  • “An easy-to-use excimer fluorescence derivatization reagent, 2-chloro-4-methoxy-6-(4-(pyren-4-yl)butoxy)-1,3,5-triazine, for use in the highly sensitive and selective liquid chromatography analysis of histamine in Japanese soy sauces” Anal. Chim. Acta, 880, 145-151 (2015).
  • “Automatic analyzer for highly polar carboxylic acids based on fluorescence derivatization–liquid chromatography” Biomed. Chromatogr., 29, 445-451 (2015).
  • “Dress-up chiral columns for the enantioseparation of amino acids based on fluorous separation” Anal. Bioanal. Chem., 405, 8121-8129 (2013).
  • “4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride as an enantioseparation enhancer for fluorescence chiral derivatization–liquid chromatographic analysis of DL-lactic acid” J. Chromatogr. A, 1360, 188-195 (2014).
  • “Simple and Sensitive Analysis of Histamine and Tyramine in Japanese Soy Sauces and Their Intermediates Using the Stable Isotope Dilution HILIC−MS/MS Method” J. Agric. Food Chem., 62, 6206-6211 (2014).
  • “Computational Prediction of Diastereomeric Separation Behavior of Fluorescent o-Phthalaldehyde Derivatives of Amino Acids” Anal. Sci. (HOT ARTICLE & Cover illustration of this month), 30, 865-870 (2014).
  • “Assessment of the Efficacy of Anticancer Drugs by Amino Acid Metabolomics Using Fluorescence Derivatization-HPLC” Anal. Sci. (HOT ARTICLE), 30, 751-758 (2014).
  • “Fully automated reagent peak-free liquid chromatography fluorescence analysis of highly polar carboxylic acids using a column-switching system and fluorous scavenging derivatization” J. Sep. Sci., 36, 232–238 (2013).
  • “Binary Fluorous Alkylation of Biogenic Primary Amines with Perfluorinated Aldehyde Followed by Fluorous Liquid Chromatography-Tandem Mass Spectrometry Analysis” Anal. Chem., 84, 8407-8414 (2012).
  • “Determination of Histamine in Microdialysis Samples from Guinea Pig Skin by High-Performance Liquid Chromatography with Fluorescence Detection” Skin Pharm. Physiol., 25, 65-72 (2012)
  • “Highly sensitive and selective derivatization LC method for biomolecules based on fluorescence interactions and fluorous separations” J. Chromatogr. B, 879, 1325-1337 (2011)(総説).
  • “Development of Novel Fluorescence-Derivatization-HPLC Methods Enabling Highly Sensitive and Selective Analysis of Biological Compounds” Yakugaku Zasshi, 131, 1207-1211 (2011)(総説).
  • “Highly sensitive capillary electrophoresis analysis of N-linked oligosaccharides in glycoproteins following fluorescence derivatization with rhodamine 110 and laser-induced fluorescence detection” Electrophoresis, 32, 3499-3504 (2011).
  • “Selective liquid-chromatographic determination of native fluorescent biogenic amines in human urine based on fluorous derivatization” J. Chromatogr. A, 1218, 5581-5586 (2011).
  • “Fluorous derivatization and fluorous-phase separation for fluorometric determination of naproxen and felbinac in human plasma” J. Pharm. Biomed. Anal., 55(1), 176-180 (2011).
  • “Liquid chromatographic determination of aminopolycarboxylic acids in water samples based on intramolecular excimer-forming fluorescence derivatization” Bunseki Kagaku, 60(1), 39-44 (2011).
  • “Selective determination of cysteines through precolumn double labeling and liquid chromatography followed by detection of intramolecular FRET” Anal. Bioanal. Chem., 399(4), 1665-1675 (2011).
  • “Development of novel fluorescence derivatization systems enabling highly sensitive and selective detection of biological compounds” Chromatography, 31(1), 23-30 (2010)(総説).
  • “Reagent Peak-Free Liquid Chromatography- Fluorescence Analysis of Carboxylic Acids Using Fluorous Scavenging Derivatization Method” Anal. Bioanal. Chem., 397, 2409-2416 (2010).
  • “Fluorous derivatization combined with LC-MS/MS: A method for the selective and sensitive determination of sialic acids in biological samples” Rapid Commun. Mass Spectr., 24, 1-7 (2010).
  • “Sensitive determination of rhodamine 110-labeled monosaccharides in glycoprotein by capillary electrophoresis with laser-induced fluorescence detection” J. Chromatogr. A, 1217, 3161-3166 (2010).
  • “A fluorous tag-bound fluorescence derivatization reagent, F-trap pyrene, for reagent peak-free HPLC analysis of aliphatic amines” Anal. Bioanal. Chem., 394(1), 321-327 (2009).
  • “Separation-oriented Derivatization of Native Fluorescent Compounds through Fluorous Labeling Followed by LC with Fluorous-phase” Anal. Chem., 81(12), 5039-5045 (2009).
  • “Liquid Chromatographic Determination of Acetylcholine Based on Pre-column Alkaline Cleavage Reaction and Post-column Tris(2,2'-bipyridyl)ruthenium(III) Chemiluminescence Detection” Luminescence, 24(5), 306-310 (2009).
  • “Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-forming Derivatization and LC with Fluorescence Detection” Chromatographia, 70(3-4), 575-580 (2009).
  • “Liquid Chromatographic Determination of Polythiols Based on Pre-column Excimer Fluorescence Derivatization and Its Application to α-Lipoic Acid Analysis” J. Chromatogr. A, 1216(44), 7564-7569 (2009).
  • “Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Post-column Excimer Fluorescence Derivatization” Anal. Sci., 25(6), 829-833 (2009).
  • “Determination of polar organophosphorus pesticides in water samples by hydrophilic interaction liquid chromatography with tandem mass spectrometry” Rapid Commun. Mass Spectr., 22(14), 2203-2210 (2008).
  • “[14C]Benzyl acetate is a potential radiotracer for the measurement of glial metabolism in the rat brain” Nucl. Med. Biol., 34(8), 939-944 (2007).
  • “Determination of catecholamines and indoleamines in human urine based on intramolecular excimer-forming derivatization and fluorescence detection” Anal. Sci., 23(4), 485-488 (2007).
  • “Selective Determination of Tryptophan-containing Peptides through Precolumn Derivatization and Liquid Chromatography Using Intramolecular Fluorescence Resonance Energy Transfer Detection” Anal. Sci., 23(8), 949-953 (2007).
  • “Simplified Method for Determination of Polycarbamate Fungicide in Water Samples by Liquid Chromatography with Tandem Mass Spectrometry Following Derivatization with Dimethyl sulfate” J. Chromatogr. A, 1141(2), 251-257 (2007).
  • “Online photocatalytic device for highly selective pre-column fluorescence derivatization of 5-hydroxyindoles with benzylamine” Anal. Chim. Acta, 555, 14-19 (2006).
  • “Simultaneous liquid chromatographic measurement of melatonin and related indoles through post-column electrochemical demethylation and fluorescence derivatization” Anal. Sci., 22(2), 281-286 (2006).
  • “Selective determination of native fluorescent bioamines through precolumn derivatization and liquid chromatography using intramolecular fluorescence resonance energy transfer detection” Anal. Chem., 78(3), 920-927 (2006).
  • “Derivatization chemistries for determination of serotonin, norepinephirine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection” Biomed. Chromatogr., 20(3), 267-281 (2006).
  • “光触媒酸化反応を利用する5-ヒドロキシインドール類のプレカラム蛍光誘導体化液体クロマトグラフィー”. 分析化学, 54(12), 1211-1214 (2005).
  • “A Simple Liquid Chromatographic Method Based on Intramolecular Excimer-forming Derivatization And Fluorescence Detection for the Determination of Tyrosine and Tyramine in Urine” J. Chromatogr. B, 821(1), 88-93 (2005).
  • “Improvement of Fluorescence Characteristics of Coumarins: Syntheses and Fluorescence Properties of 6-Methoxycoumarin and Benzocoumarin Derivatives as Novel Fluorophores Emitting in the Longer Wavelength Region and Their Application to Analytical Reagents” Chem. Pharm. Bull., 53(7), 750-758 (2005).
  • “Determaination of tetrabromobisphenol A in human serum by liquid chromatography-electrospray ionization tandem mass spectrometry” J. Chromatogr. B, 809, 131-136 (2004).
  • “Liquid Chromatographic Determination of Ethambutol in Serum Samples Based on Intramolecular Excimer-forming Fluorescence Derivatization” Anal. Sci., 20, 489-493 (2004).
  • “Rhodamine B amine as a highly sensitive fluorescence derivatization reagent for saccharides in reversed-phase HPLC” J. Chromatogr. A, 1038, 113-120 (2004).
  • “Selective Determination of Tetrabromobisphenol A by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent” Organohalogen Compounds, 66, 315-320 (2004).
  • “Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent” Anal. Sci., 20, 557-559 (2004).
  • “A sensitive and selective determination method of histamine by HPLC with intermolecular excimer-forming derivatizatiion and fluorescence detection” Biomed. Chromatogr., 17 (8), 509-516 (2003).
  • “Determination of Midodrine in Human Plasma by High-performance Liquid Chromatography with Fluorescence Detection” Anal. Sci., 19, 317-319 (2003).
  • “Highly selective fluorimetric determination of acidic amino acids by high-performance liquid chromatography following intramolecular excimer-forming derivatization with pyrene-labeling reagent” BUNSEKI KAGAKU, 52 (12), 1113-1119 (2003).
  • “Chemiluminescence Characteristics of N-(4-Substituted Benzyl)isoluminol”, Chem. Pharm. Bull., 48, 2011-2013 (2000).

○著書
  • 「アップデート薬学機器分析学」,廣川書店,轟木堅一郎,明樂一己 編集,編集および第1章,第2章を分担執筆
  • “Amino acid and bioamine separations, Handbooks in Separation Science Liquid Chromatography, 2nd Edition, Elsevier, 87-106 (2017)
  • 「パートナー分析化学II」,南江堂,山口政俊,升島 努,能田 均 編集,第5章免疫測定法(p259-272)を分担執筆
  • 「演習で理解する薬学の分析化学」,廣川書店,山口政俊,鶴田泰人,能田 均 編集,第8章光分析法(p133-154)を分担執筆
  • 「蛍光分子間相互作用を利用する高選択的分析法」,じほう社,日本薬学会物理系薬学部会・分析化学担当教員会議編「薬学分析科学の最前線」の第4章の一部(p52-53)を分担執筆
  • 「微小透析・蛍光HPLCによる脳内アミンのインビボ計測」,じほう社,日本薬学会物理系薬学部会・分析化学担当教員会議編「薬学分析科学の最前線」の第5章の一部(p94-95)を分担執筆

教育・研究に対する考え方

教育:分析化学は,科学計測を必要とする全ての自然科学において根幹をなす重要な学問である。一方で,現在の分析機器は試料をセットするだけで測定結果が数値やスペクトルとして簡単に得られることも多く,その結果が信用たるものかを判断するためには,用いた分析機器がどのような原理に基づいて測定しているのか,あるいは測定結果に影響を及ぼす因子が何であり,それが適切に除外できていたのかなど,分析化学に関する本質的な理解が不可欠である。そのため,講義では本質的な理解に繋がるよう理論と計算を特に重視し,演習により知識の定着を図る。
研究:研究指導においては,各自が1研究者として主体的に研究を進めてもらうべく個別にテーマを与え,ディスカッションを中心とした研究指導を行う。また早期から積極的に学会発表させることで,自身の研究の立ち位置と到達目標を明確にし,多くの研究者と交流させることで彼らの意識と能力の向上に努める。

研究シーズ集に関するキーワード

クロマトグラフィー,蛍光分析,抗体医薬分析,フルオラス化学,質量分析

モバイル表示

PC表示